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From assay miniaturization to scale: Automating AlphaLISA with firefly®

In this webinar, Katherine Dyer of Transcripta Bio and Dr. Kyle Kisor of SPT Labtech will demonstrate how they developed and automated an AlphaLISA workflow on the firefly® liquid handler, from initial miniaturization to full-plate implementation.

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Webinar
dragonfly discovery - Precision dispensing complete eBook

Precision dispensing for every stage of discovery. One platform. Multiple workflows. Endless possibilities.

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Guide
dragonfly® discovery: Assay development meets uHTS

Ultra-high-throughput screening (uHTS) demands homogeneous assay formats to leverage the density of 1536/3456 well plates to increase the throughput of large compound (> 1 million) library screens against disease targets. Such assay formats require automated reagent dispensing and rigorous assay development. The key liquid handlers in any uHTS system are non-contact reagent dispensers rather than tip-based multichannel pipetting devices that are common in many lower throughput assay systems. Critical requirements for any uHTS reagent dispenser are accuracy, precision, and speed. Additionally, it is important to integrate the uHTS dispenser into assay development as early as possible. For most bulk dispensers, this integration typically occurs late in the process. The dragonfly discovery from SPT Labtech is a non-contact positive displacement dispenser designed to bridge dispensing needs from assay development through high-throughput screening. It provides ready access to 10 different reagents that can be dispensed in variable volumes as well as at high speeds. We evaluate the performance of this dispenser in high-throughput mode and examine its use in assay development for reagent concentration optimization in 1536-well plates and buffer component optimization by DOE.

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Poster
The application of Design of Experiment and the dragonfly discovery to miniaturise, automate and accelerate assay optimization

Automation to carry out complex DoE experiments has always been a limiting factor, both in terms of speed and cost. The dragonfly discovery from SPT Labtech, is a rapid and reliable low volume liquid handler that employs true positive displacement technology to enable the dispensing of all aqueous solutions and solvents, from 200nL through to 4mL. The dragonfly discovery's plate-map file format made it simple to convert and import JMP DoE files and subsequently execute these experimental designs in all microplate types, including 1,536 well plates.

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Poster
dragonfly discovery to enable and accelerate early stage drug discovery processes

The complexity of experimental design in early stage drug discovery is rapidly increasing with reagent requirements for a growing number of assays becoming progressively more complex and difficult to dispense. In order to address these challenges, we have utilized the dragonfly discovery to enable a broad range of applications within functional genomics, phenotypic and biochemical assay workflows.

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Poster
Reimagining Adherent Cell Assays: Flexible Automation with firefly and SemaCyte Technologies

In this joint webinar, Semarion and SPT Labtech walk through the development of robust, automated protocols for dispensing and processing SemaCyte-loaded microwells with minimal manual intervention. Using the firefly, all-in-one liquid handler, the team demonstrates how end-to-end assay automation becomes practical and scalable.

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Webinar
firefly Demo Overview

Watch as firefly effortlessly streamlines workflows, reducing human error and increasing throughput. With its advanced technology and user-friendly interface, you can focus on your research while firefly takes care of the details. Discover the future of laboratory automation.

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Video
Optimizing multi-cellular models for high-throughput screening - Webinar

Join us as we dive into recent research which has combined Design of Experiments methodology with automated liquid handling to develop a cardiotoxicity assay platform. We discuss how this approach could become routine in the future, not only offering more accurate predictability of new drug candidates, but also enabling researchers to leverage resources more efficiently through streamlined workflows and data-driven decision-making.

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Webinar
How to accelerate small molecule lead discovery with automated multichannel pipetting and DEL technology

Establishing an efficient and accurate compound testing workflow is essential to any small molecule screening platform. When utilizing screening methods such as DNA-encoded library (DEL) technology, follow-up hit validation must be performed using traditional biochemical assays. In this webinar, we will review the use of mosquito LV to accelerate early-stage small molecule lead discovery through the lens of Hydroxyacid oxidase 1 (HAO1). In this case study, we will describe our efforts to develop and deploy a robust enzymatic assay towards the development of potent inhibitors identified by DEL technology for disease treatment.

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Webinar
apricot S3 - An overview with Johnathan Faherty

An overview of the apricot S3 with our product manager Jonathan Faherty. Jonathan explains a better way to carry out your serial dilutions with the apricot S3.

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Video
mosquito® LV: An Assay’s Best Friend

After a successful DNA-encoded library (DEL) screen, it is essential to have access to a high-throughput method of assaying compounds to validate hits. The ability to create up to sixteen serial dilutions in parallel and subsequently plate those dilutions on a nanoscale can vastly speed up the hit finding process, while improving the accuracy and efficiency of the screen. mosquito® LV by SPT Labtech has greatly improved the way we work with our off-DNA compounds synthesized from DELcore and DELflex collaborations.

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Application note
The Future Is Here: Precision Automation Demonstrates Its Value as a Tool for Accelerating Drug Discovery

With more than 70 FDA-approved small molecules ,kinase inhibition continues to attract significant investment. Identification and characterization of novel inhibitors is a challenging, yet critical task which sets the tone for future successes and failures. Traditional single shot approaches do not meet current requirements for machine learning and carry significant risks of false negatives. Hit profiling has evolved this concept, generating potency estimates, reducing risks while providing contextual confidence. Accurate hit identification followed by high resolution, kinetic molecular profiling enhances outcomes ensuring better data-driven decision-making earlier in the process, saving time and resources, leading to superior molecular design.

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Poster
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