firefly features a 384 pipetting module and 3 or 6 dispense heads to provide flexible high performance approaches to a range of applications such as NGS library prep and PCR set up. An ingenious 2 tier moving deck system with an additional sub-deck layer for accessories packs a lot of capability into a very small footprint.
Pipetting head operates in several configurations: 384, 96, columns of 16 (384 format) and 8 (96 format) from 0.5uL to 125uL (extending to 500uL with optional adapter). Utilizes robust and proven air displacement technology with disposable tips and a range of tip types including sterile and filtered options.
Dispense heads provide either 3 or 6 positive displacement syringes for non-contact, liquid agnostic reagent dispensing. Syringes can operate completely independently for multiple reagent dispensing or in parallel (any combination) for speed.
The gripper module works in combination with the pipetting head and can move labware between the decks and sub-deck levels.
Optional application accessories provide greater walk-away capability with options for magnetic plates, cooling and heating tiles, and shakers.
The safety enclosure protects users from moving parts while protecting samples and reagents from airborne contaminants. A large interlocked door provides safe and easy access to load the system.
firefly sets a new standard in genomics liquid handling software with easy accessible user interface, secure log-in and audit trails, multiple user levels and intelligent programming. With cloud-based sharing and the ability to author on any PC, firefly software makes automation accessible to any lab user.
The graphical interface is extremely intuitive and accessible to users of all levels. Researcher scan quickly build new protocols on any Windows PC or compatible device, even in the coffee shop! Simple drag and drop enables easy protocol set up with accessible extended features for fine tuning by advanced users. Protocols can also be locked down and tailored to provide the simplest set up for any user with clear running instructions to make the system truly accessible to anyone in the lab. Powerful audit trails and user access also ensure that methods are protected and provide tools to aid compliance in regulated environments.
firefly can be programmed and protocols executed at the instrument through the integrated tablet. The software can also be installed on external Windows-based devices to enable protocol writing away from the system. Easily share protocols with the instrument via the cloud connection, network or even a USB stick providing ultimate flexibility and convenience.
firefly software provides cloud access to a user community enabling the sharing of protocols between users and access to SPT labtech validated protocols and tools.
The pipetting head has a 384 core capable of parallel pipetting up to 384 individual samples between 0.5uL and 125uL (extended to 500uL with adaptor plate in 96 format only). Disposable tips are available in other formats to support working in both 384 and 96 well labware.
- 384 standard, sterile, filtered and sterile; 125uL, 50uL and 15uL
- 96 standard, sterile, filtered and sterile; 125uL, 50uL and 15uL
- 8 strips (96 format) standard, sterile, filtered and steril; 125uL, 50uL and 15uL
- 16 strips (384 format) standard, sterile, filtered and steril; 125uL, 50uL and 15uL
Strip tips enable users to work with partial plates or across plates to facilitate processes such as serial dilutions
An optional adaptor plate extends the pipetting range to 500uL (96 format only)
The system is equipped with either 3 or 6 dispensing heads which can be synchronized for high throughput or controlled independently for reagent flexibility.
True positive displacement disposable syringes provide liquid class agnostic non-contact dispensing of volumes from 200nL to 4mL. Each syringe can be used as a 4mL reservoir to multi-dispense smaller volumes, dispensing to a whole 96 or 384 well plate in as little as 30 seconds.
The non-contact dispensing mechanism enables tips to be used for multiple operations per reagent and dramatically reduces tip consumption versus standard pipetting approaches. No calibration, cleaning, or maintenance is required and there is zero chance of blocking or clogging.
The pipetting head includes a gripper module capable of moving labware between the deck and sub deck layers and moving labware on and off functional modules such as shakers and heater/cooler plates as well as magnetic blocks.
The gripper can move certain accessories such as incubation adaptor plates, so a microplate being maintained below deck on a heater/cooler plate can be moved to deck on its adaptor plate to maintain temperature whilst on the deck for pipetting or dispensing, before being returned to the heater/cooler to return to active temperature control.
The gripper is capable of moving labware with pipette tips in place for increased workflow efficiency, alternatively tips can be removed before working with the gripper. It moves in tandem with the pipetting head in terms of Y and Z movement as well as having a further Z motion so that it can reach down below the pipette tips to grip labware. The grip mechanism can be trained to pick and place a wide variety of ANSI/SBS labware.