Resources

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KAPA EvoPrep Automated Library Preparation - firefly Technical Note

This technical note provides supporting information for the firefly protocols listed below. These protocols are based on the KAPA EvoPrep Kit Operators Manual v1.2 and are available to download from the firefly community. Here, we outline protocol run times, parts required and provide details on the steps performed in each protocol.

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Technical note
Automating KAPA EvoPrep Library Preparation on firefly®

The KAPA EvoPrep Kits are the latest high-performance, streamlined, and automation-friendly library preparation solutions from Roche. SPT Labtech firefly protocols have been developed to run the KAPA EvoPrep and KAPA EvoPrep PCR-Free library preparation workflows - using the 96-well plate format of the KAPA EvoPrep Kit and the KAPA Unique Dual-Indexed Adapter Kit.

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Application note
Technical Note - NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina

This technical note provides supporting information for automation of the NEBNext Ultra II FS DNA Library Prep kit on firefly. The firefly protocols are listed below and are available to download from the firefly community. Here, we outline protocol run times, parts required and provide details on the steps performed in each protocol.

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Technical note
Application Note - Automating the NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina on firefly®

The NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina is optimized for the construction of high quality, high yield libraries using a broad range of DNA input amounts. The library preparation workflow uses NEBNext FS (Fragmentation System) enzymatic fragmentation reagents, eliminating the need to mechanically shear the DNA input. The workflow also uses a single enzyme mix to perform the fragmentation, end repair and dA-tailing, which greatly simplifies the workflow. Here, we demonstrate how this workflow has been automated on firefly. We provide details on the steps performed in each protocol, together with data to demonstrate the performance of these protocols in generating high quality libraries for sequencing.

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Application note
Advancing 3D Cell Culture: Automated positive displacement dispensing of MCF7 and HepG2 cells, mixed with PeptiMatrix™ Hydrogel

In vitro cell cultures are essential for modelling disease, but traditional 2D cell culture systems fall short in replicating the complex microenvironments of native tissues. This limitation hinders the translation of in vitro findings to in vivo contexts and contributes to the high attrition rates seen in drug discovery pipelines.

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Application note
Webinar recording: Automated sample management for more efficient drug discovery: Developing and embedding in-house sample management at Artios

In this webinar, learn how the team at Artios Pharma have developed and implemented automated end-to-end workflows for sample storage and tracking, processing, assay-ready plate generation and sample mapping for analysis, using Titian Sample Bank software and SPT Labtech automation systems. Using this compound management workflow, they have demonstrated up to a 40% reduction in data posting cycle times and decreased running costs, as well as using the system for clinical sample supply and tracking, all of which results in quicker data reporting to drive decision making and more effective use of resources.

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Webinar
High-throughput endometrial organoid plating in Matrigel using SPT Labtech's firefly®

Organoids are biologically complex three-dimensional structures that provide physiologically relevant models for studying multifaceted phenomena, such as disease progression, embryonic development, and drug response. The use of organoids has been steadily increasing and triggers the demand for reliable and scalable workflows for their generation and culture. Organoids typically require a supportive matrix that facilitates the formation of three-dimensional structures and sustains their proliferation.

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Application note
Efficient Long-Read Genome Library Prep in the Wellcome Sanger Institute’s Darwin Tree of Life Project

Presenting the successful automation of a long-read whole genome library preparation using the PacBio SMRTbell long-read prep kit 3.0 (SPK3) on SPT Labtech's firefly liquid handling platform.

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Webinar
BioMicroLab XL9 & XL20 Tube Handling Systems Brochure

The XL9 & XL20 are reliable, flexible and affordable robotic pick and place systems used to automate critical sample management tasks such as organizing, reformatting and accurately tracking samples. The XL9 and XL20 are fully compatible with LIMS systems and with multiple labware formats. Choose and order your XL Series platform with the capacity and modules right for your lab’s application.

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Brochure
Automating antibiotic discovery in academia: A collaborative screening effort to identify novel TB therapeutics

In this webinar, hear how a collaboration between SPT Labtech, Automata, GSK, and researchers at the Francis Crick Institute, employed cutting-edge automation tools, including the dragonfly® discovery liquid handling system by SPT Labtech and Automata’s LINQ platform, to conduct a high-throughput enzymatic screen targeting a vulnerable pathway in Mycobacterium tuberculosis (Mtb).

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Webinar
An introduction to mosquito Gen3 - Product Overview

Introducing our most compact and accessible member of the mosquito family. mosquito Gen3 offers highly accurate and precise multi-channel pipetting from 500 nL to 5 µL, allowing genomics researchers to benefit from miniaturization across a range of applications, irrespective of liquid class. The instrument’s rapid plate-to-plate pipetting motion and tip exchange maximize speed and efficiency.

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Video
Automating NEBNext® UltraExpress® NGS Library Prep with SPT Labtech’s firefly® for rapid, high-throughput NGS library generation at Harvard Medical School’s core facility

We demonstrate the ease of automating NEB’s UltraExpress® kits with the user-friendly firefly® system, achieving high-quality and reproducible libraries and sequencing data. Libraries were prepared from 25ng to 250ng of total RNA, consistently showing high data quality across this input range.

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Poster
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