Resources

Explore insightful resources that include videos, whitepapers, eBooks, application notes and more

Scaling up plate-based single-cell profiling: introducing automated STORM-seq

Full-length, single-cell RNA-seq (scRNA-seq) allows for more complete transcriptomic profiling compared to 5' or 3' end-counting methods. However, labor-intensive, non-modular workflows with high reagent costs limit scalability. Advancements in laboratory automation, protocol design, and reaction miniaturization have overcome some of these constraints. In collaboration with the Van Andel Institute, we present Single-cell TOtal RNA Miniaturized sequencing (STORM-seq), which pairs Takara Bio’s SMART® technology with SPT Labtech’s mosquito® HV genomics liquid handling platform. This miniaturized, plate-based scRNA-seq approach is scalable, automated, and offers a solution for single-cell transcriptomic profiling (i.e., coding/noncoding transcripts, isoforms, gene fusions, etc.) at reduced cost and increased cell throughput.

Read more
Poster
Scaling single-cell RNA sequencing with automated liquid handling

The ability to obtain and map transcripts from single cells has provided significant insight into the fundamentals of how life persists and how disease evolves. While their plate-based workflows have increased accessibility significantly without requiring specialized instruments, automation of these methods can significantly reduce consumable consumption and streamline processing time while reducing user pipetting errors and strain. This poster outlines the approach, benefits, and results of automating the Scale Biosciences Single Cell RNA Sequencing Kit on SPT Labtech’s all-in-one liquid handling platform, firefly®.

Read more
Poster
Transforming PCR protocols in pandemic response with automated non-contact liquid dispensing

The UK’s first testing “megalab”, the Rosalind Franklin Laboratory (RFL) in Royal Leamington Spa, was established in 2021 with the aim of processing and sequencing thousands of COVID-19 tests a week, playing a key role in preventing disease outbreaks and responding to new variants of concern and future disease threats. With the critical need for accurate pathogen surveillance, the manual execution of PCR workflows emerged as a bottleneck, limiting throughput and introducing potential for human error and variability among lab personnel. To enhance efficiency and data quality, the lab chose to adopt dragonfly® discovery to improve the accuracy and reliability of extraction plate preparation for the UltraDX SARS-CoV-2 N1/N2/RP assay (LGC, Biosearch Technologies).

Read more
Poster
Automating Scale Biosciences’ Single Cell RNA Kit on SPT Labtech’s firefly®

The ability to obtain and map transcripts from single cells has provided significant insight into the fundamentals of how life persists and how disease evolves. ScaleBio enables single cell analysis with an instrument-free method leveraging highly parallelized barcoding, making single cell RNA sequencing more approachable for researchers across both academic and industry spectrums.

Read more
Application note
dragonfly discovery technical note: PCR reaction set-up by dragonfly®

A vast number of advantages can be gained by semi-automating genomics workflows through use of low volume liquid handling instruments, such the mosquito® genomics and dragonfly® discovery offered by SPT Labtech. Benefits include the miniaturization of reaction volumes to reduce cost per sample and the elimination of most sources of human error to increase experimental reproducibility. In this technical note we have tested the effect of mixing on cross-contamination between different wells during PCR set-up.

Read more
Technical note
firefly technical note: KAPA EvoPlus Automated Library Preparation

This technical note provides supporting information for automating the KAPA EvoPlus DNA Library Prep Kit on SPT Labtech's firefly liquid handler. These protocols are available to download from the firefly community. Here we provide information on protocol run times, consumables usage and present data generated using these protocols.

Read more
Technical note
Fully automated bead-based High Molecular Weight DNA extraction for long-read sequencing

The growing popularity of long read sequencing (LRS) is driving up demand for High Molecular Weight (HMW) DNA. However, the extraction of HMW DNA has proven to be a barrier for routine uses. Many conventional methods are expensive, resource- and time-intensive, and do not produce DNA of sufficient quantity and quality for LRS. Moreover, they might be challenging to scale up due to not being automation-friendly or requiring specialized equipment and labware, like vacuum manifolds or wide bore tips, driving the costs up.

Read more
Application note
Enhancing Core Facility Operations: Automated Liquid Handling for High-Throughput, High-Quality NGS Library Generation

In this webinar, researchers from the Biopolymers Genomics Core Facility (BPF) at Harvard Medical School will discuss automating the versatile NEBNext UltraExpress DNA Library Prep Kit using SPT Labtech’s firefly liquid handling platform to generate high-quality libraries for next-generation sequencing from a broad input range of DNA. The accessible, streamlined NEBNext UltraExpress workflow in tandem with user-friendly firefly creates accessible means for generating NGS libraries for Illumina DNA sequencing. The speakers will explain how the ease of use of the firefly allowed the NEBNext UltraExpress kit to be easily written and optimized for automation and to facilitate a more productive sequencing service.

Read more
Webinar
Accelerating Drug Discovery at Totus Medicines: DNA-Encoded Library Screening with firefly®

DNA-encoded libraries (DELs) have emerged as a transformative technology within drug discovery. The exploration of large libraries of small molecule ligands as a collective rather than an individual has springboarded DELs to the forefront of high throughput screening. Building upon traditional DEL methods, Totus Medicines has developed a platform capable of identifying covalent small molecule ligands via high-throughput cell-based DEL screening. This poster outlines how Totus Medicines is streamlining hit identification, optimization, and technology development, to accelerate the discovery of novel therapeutic candidates using SPT Labtech’s firefly automated liquid handling platform. We present the metrics that have been collected over one year of screening using Totus Medicine’s proprietary covalent DELs to run over 2000 unique selections against a large number of targets, spanning diverse therapeutic areas.

Read more
Poster
dragonfly® discovery: Pioneering Sustainable Liquid Handling in the Modern Laboratory

In this poster, we highlight the significant role that dragonfly discovery plays in reducing the environmental impact of liquid handling workflows. In our first case study, dragonfly discovery demonstrates a reduction in plastic pipette tip usage by over 99% compared to traditional air displacement pipetting systems when performing a 3456-run enzymatic assay operated by 6-factor space-filling Design of Experiment (DoE). In Next-Generation Sequencing (NGS) library preparation workflows, such as the NEBNext® Ultra™ II FS DNA Library prep kit presented in our second case study, dragonfly discovery achieves a plastic reduction of over 50%.

Read more
Poster
Towards high-throughput low-cost sustainable ecotoxicity testing

Traditional whole animal-based toxicity testing is expensive, time-consuming, and unethical. Furthermore, these tests do not capture the full range of biological impacts. There is growing interest in developing alternative methods (New Approach Methods, NAMs), that are less resource intensive (lower costs, quicker assays, fewer animals), while also providing more data on the mechanism of toxicity. The objective of this work was to optimize and establish a high throughput in vitro transcriptomic-based toxicity testing platform for chemical and environmental risk assessment. The platform couples human Caco-2 and Hep G2 cell lines with QIAGEN’s UPXome ultraplex technology, so that the combined test system can also derive transcriptomic points of departure (tPODs).

Read more
Poster
Auto-tRNAseq: Driving throughput in tRNA library generation with firefly®

Transfer RNAs (tRNAs) are the critical adaptor molecules that translate genetic information into functional protein sequences. While the genome defines a static set of tRNA gene sequences, the functional tRNA pool depends on the expression and aminoacylation levels of different tRNA species, as well as many modifications that fine-tune tRNA activity.1,2 Dynamically regulated tRNA pools dictate the fidelity of the translation process and proteome integrity of a cell. Considering the central role of tRNA in translation regulation, investigating the cellular composition of tRNA pools provides a deeper understanding of the translational state of the cell. High-resolution sequencing-based methods for tRNA sequencing have provided transformative insights into the physiological impact of tRNA expression, modification, and aminoacylation levels on cellular stress and/or disease conditions.3–6 More recently, large-scale genome engineering efforts have also employed tRNA sequencing to recapitulate the translational state of engineered cells.

Read more
Poster
Page 1 of 22