Cryo-EM has developed into a powerful tool for studying the fine detail of biological structure required in areas such as drug discovery. Despite this, producing quality frozen grids with vitreous ice of an appropriate thickness and evenly distributed particles remains a key bottleneck in the structure determination workflow.
We drew on decades of experience in structural biology process automation to create chameleon and provide optimizable vitrification for each unique sample behavior, reducing the time and costs associated with traditional trial-and-error methods.
"The chameleon has enabled an efficient cryoEM workflow for Arvinas by allowing us to freeze our samples promptly upon elution from the column, thus allowing rapid capture of complexes on grids ready for cryoEM data collection. By improving sample quality and reducing screening loads on microscopes, chameleon has allowed us to leverage both academic and industry partners for data collection."
chameleon streamlines cryo-EM sample preparation workflows with high speed blot-free vitrification, automated grid handling, and the ability to screen grids based on likely ice thickness. Guided workflows simplify set up, use, and cleaning of the instrument while the minimization of manual handling eliminates sample loss and damage.
chameleon delivers optimized grid vitrification for cryo-EM by combining next-generation automation, blotless grid technology, and high speed plunging. Thinking beyond existing sample preparation workflows enables routine high resolution structural studies.
QUANTIFOIL Active grids are unique in being the first commercially produced grids with copper nano-wire covered bars to promote thin film formation through a process of “self-wicking”. This novel grid technology removes the variability of applying an externally applied filter paper to remove excess sample during blotting.
Cryo-EM has been adopted into drug discovery workflows by the pharmaceutical and biotechnology industries having proved itself capable of delivering atomic resolution structures for key targets, including integral membrane proteins such as GPCRs and ion channels.
The NIS and SPT Labtech expert discussion will be recorded at the Microscopy & Microanalysis Meeting 2022 in Portland and aims to cover key questions around best practice for dealing with sample preparation issues.
In this presentation with MIT.nano, SPT Labtech's cryo-EM development Scientist Michele Darrow discusses using new automation to overcome commonly experienced sample preparation bottlenecks ahead of the microscope.