Dynamic duo for miniaturizing NGS methods

      Increase your productivity and reduce the cost per sample by miniaturizing your NGS method with proven workflow solutions for over 40 NGS kits, including industry standards such as Illumina Nextera XT, Illumina DNA Prep, NEBNext Ultra II, plus many more.

      dynamic-duo-banner-v4 (1)

      Illumina, PacBio, Nanopore … boost the productivity of your NGS workflows

      Volume miniaturization with our automated liquid handlers – mosquito® and dragonfly® – solves throughput and cost challenges, enabling genomics researchers to process more samples and make savings on reagent use.

      Our 'dynamic duo' combines mosquito's highly accurate positive displacement pipetting with dragonfly's non-contact, nanoliter-to-milliliter dispensing. mosquito gives you full visibility with the ability to aspirate, dispense, mix, and perform on-plate serial dilutions, while dragonfly delivers accurate nanoliter dispensing irrespective of liquid viscosity or environmental conditions. Increase the productivity of your workflow with successful miniaturization solutions for a growing list of NGS methods and qPCR/PCR setups.

      See the links to resources and customer success stories in the methods table below: over 40 NGS methods successfully miniaturized​.

       

      mosquito® HV genomics

      mosquito HV genomics offers highly accurate and precise multi-channel positive displacement pipetting from 500 nL to 5 µL allowing genomics researchers to benefit from miniaturization and automation across a range of applications, irrespective of liquid class. The instrument’s pre-sterilized disposable pipettes eliminate cross-contamination and maintain data integrity while the rapid plate-to-plate pipetting motion and tip exchange maximize speed and efficiency

      Explore mosquito HV genomics
      mosquit-hv-genomics-right
      mosquit-lv-genomics-left

      mosquito® LV genomics

      mosquito LV (Low Volume) genomics offers highly accurate and precise multi-channel positive displacement pipetting from 25 nL to 1.2 μL. It enables miniaturization of a wide range of genomics applications, delivering a step change in productivity, while reducing costs.

      Explore mosquito LV genomics

      dragonfly® discovery

      dragonfly discovery enables innovative, low volume reagent dispensing across a broad range of applications including genomics​, assay development, and 3D cell cultures. Highly repeatable and accurate low volume dispensing, rapid large volume dispensing, and ultra-low dead volumes allow researchers to minimize reagent costs, save time, and standardize assays for superior quality data.

      Explore dragonfly discovery
      dragonfly-product-category---product-dfdiscovery

      Workflow examples

      Click on the workflow to expand.

      Over 40 NGS methods miniaturized​

      *Scroll left and right to view more information

      Application Area

      Kit Name

      Instrument Model

      Standard Volume [µL]​

      Miniaturized Volume [µL]​

      N-Fold

      Citations (hover for resource name)

      SINGLE CELL/LOW INPUT CDNA SYNTHESIS ​(full transcriptome)

      SMART-Seq2

      mosquito HV genomics mosquito LV genomics dragonfly discovery

      25

      2.5

      Up to 10x

      SMART-Seq V3

      mosquito HV genomics

      7

      3.5

      Up to 2x

      Takara SMART-Seq v4

      mosquito HV genomics

      50

      12.5

      4x

      Takara SMART-Seq Single Cell

      mosquito HV genomics

      50​

      7

      ~7x

      Takara SMART-Seq Stranded​

      mosquito HV genomics

      100

      25

      4x

      SINGLE CELL/LOW INPUT CDNA SYNTHESIS​(3’ counting)

      CEL-seq2

      mosquito LV genomics

      12

      2.6

      ~5x

      Ribo-seq

      mosquito LV genomics

      5.79

      5.79

      -

      SORT-seq

      mosquito LV genomics

      26

      2.6

      -

      SINGLE CELL/LOW INPUT WHOLE GENOME AMPLIFICATION

      Qiagen REPLI-g Single Cell​

      mosquito LV genomics

      50

      1

      50x

      DigiPico

      mosquito LV genomics

      6.2

      6.2

      -

      DNA SEQUENCING

      Illumina Nextera XT

      mosquito HV genomics mosquito LV genomics

      50

      2

      Up to 25x

      Illumina DNA Prep

      mosquito HV genomics dragonfly discovery

      50

      5

      Up to 10x

      Invitrogen Collibri ES DNA

      mosquito HV genomics

      50

      5

      10x

      NEBNext Ultra II FS DNA

      mosquito HV genomics

      50

      5

      10x

      KAPA HyperPlus

      mosquito HV genomics

      100

      10

      10x

      modified Twist Library Preparation EF Kit

      mosquito HV genomics

      48.75

      4.9

      Up to 10x

      RNA SEQUENCING

      NEBNext Ultra II Directional RNA

      mosquito HV genomics

      50

      5

      10x

      NEBNext Multiplex Small RNA

      mosquito LV genomics

      100

      20

      5x

      Takara SMARTer Stranded Total RNA-Seq

      mosquito HV genomics

      100

      12.5

      8x

      AMPLICON SEQUENCING

      COVID-seq

      mosquito HV genomics dragonfly discovery

      50

      25

      2x

      Illumina 16S Library Prep

      mosquito HV genomics

      50

      5

      10x

      Ion AmpliSeq Library Kit 2.0

      mosquito HV genomics mosquito LV genomics

      30

      2.4

      Up to 12.5x

      SAMPLE AND LIBRARY BEAD CLEAN-UP, QUANTIFICATION

      Beckman AMPure XP, SPRIselect

      mosquito HV genomics dragonfly discovery

      variable

      >10x

      Up to 10x

      Biotium AccuClear

      mosquito HV genomics mosquito LV genomics

      variable

      variable

      Up to 15x

      Invitrogen Collibri Library Quant

      mosquito HV genomics

      20

      2.5

      Up to 8x

      Promega QuantiFluor

      mosquito HV genomics mosquito LV genomics

      variable

      variable

      Up to 30x

      Quant-iT PicoGreen

      mosquito HV genomics mosquito LV genomics

      variable

      variable

      Up to 150x

      Roche KAPA Library Quant

      mosquito HV genomics

      20

      2.5

      Up to 8x

      qPCR/PCR SETUP

      gene expression profiling (Fluidigm Biomark HD)

      mosquito HV genomics

      23

      -

      -

      other

      mosquito HV genomics mosquito LV genomics

      variable

      variable

      Up to 50x

      OTHER

      Olink Explore

      mosquito LV genomics dragonfly discovery

      19.8

      -

      -

      HiFiViral SARS-CoV-2

      mosquito LV genomics dragonfly discovery

      -

      -

      -

      mosquito-product-LCP---testimonials-3

      Software that's intuitive by design

      The liquid handling software that controls the mosquito family of instruments gives users an intuitive experience tailored to their application area.

      Developed in collaboration with scientists, the latest mosquito software facilitates miniaturization in novel application areas. Packed with powerful features, the software includes an experiment designer and helpful wizards to create and manage your protocols.

      Learn more about the mosquito software