Paper
Miniaturization of Smart-seq2 for Single-Cell and Single-Nucleus RNA Sequencing
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Save up to 60% on your annual reagent costs by leasing our mosquito HV genomics automated pipettor with quarterly payments. This proven technology has successfully miniaturized over 40 NGS kits, including industry standards such as Illumina Nextera XT, Illumina DNA Prep, NEBNext Ultra II, plus many more.
We've heard you - we know that laboratories are under pressure to deliver more for less. That's why we've introduced our brand new leasing scheme.
By removing the financial barrier to lab automation, more laboratories can profit from the substantial net cost savings offered by scaling down reaction volumes, even when considering the lease payments. Miniaturization leverages the powerful automated liquid handling capabilities of mosquito to solve throughput and efficiency challenges, enabling genomics researchers to get more from precious samples and reagents. Our true positive displacement technology allows fast implementation of NGS methods so routine processing samples can be achieved in a couple of months.
With an initial commitment of just 12 months, our scheme provides you with the flexibility to adapt to your business and budget needs, to ensure you're always getting the most for your money.
It's not just us who believe in the power of our miniaturization technology - mosquito has delivered successful miniaturized solutions to a growing list of NGS methods and qPCR/PCR setups. See the links to resources and customer success stories in the methods table below: over 40 NGS methods successfully miniaturized.
mosquito HV genomics offers highly accurate and precise multi-channel positive displacement pipetting from 500 nL to 5 µL allowing genomics researchers to benefit from miniaturization and automation across a range of applications, irrespective of liquid class. The instrument’s pre-sterilized disposable pipettes eliminate cross-contamination and maintain data integrity while the rapid plate-to-plate pipetting motion and tip exchange maximize speed and efficiency
Explore mosquito HV genomics*Scroll left and right to view more information
Application Area
Kit Name
Instrument Model
Standard Volume [µL]
Miniaturized Volume [µL]
N-Fold
Citations (hover for resource name)
SINGLE CELL/LOW INPUT CDNA SYNTHESIS (full transcriptome)
SMART-Seq2
25
2.5
Up to 10x
SMART-Seq V3
7
3.5
Up to 2x
Takara SMART-Seq v4
50
12.5
4x
Takara SMART-Seq Single Cell
50
7
~7x
Takara SMART-Seq Stranded
100
25
4x
SINGLE CELL/LOW INPUT CDNA SYNTHESIS(3’ counting)
CEL-seq2
12
2.6
~5x
Ribo-seq
5.79
5.79
-
SINGLE CELL/LOW INPUT WHOLE GENOME AMPLIFICATION
Qiagen REPLI-g Single Cell
50
1
50x
DigiPico
6.2
6.2
-
DNA SEQUENCING
Illumina Nextera XT
50
2
Up to 25x
Illumina DNA Prep
50
5
Up to 10x
Invitrogen Collibri ES DNA
50
5
10x
NEBNext Ultra II FS DNA
50
5
10x
KAPA HyperPlus
100
10
10x
modified Twist Library Preparation EF Kit
48.75
4.9
Up to 10x
RNA SEQUENCING
NEBNext Ultra II Directional RNA
50
5
10x
NEBNext Multiplex Small RNA
100
20
5x
Takara SMARTer Stranded Total RNA-Seq
100
12.5
8x
AMPLICON SEQUENCING
COVID-seq
50
25
2x
Illumina 16S Library Prep
50
5
10x
Ion AmpliSeq Library Kit 2.0
30
2.4
Up to 12.5x
SAMPLE AND LIBRARY BEAD CLEAN-UP, QUANTIFICATION
Beckman AMPure XP, SPRIselect
variable
>10x
Up to 10x
Biotium AccuClear
variable
variable
Up to 15x
Invitrogen Collibri Library Quant
20
2.5
Up to 8x
Promega QuantiFluor
variable
variable
Up to 30x
Quant-iT PicoGreen
variable
variable
Up to 150x
Roche KAPA Library Quant
20
2.5
Up to 8x
qPCR/PCR SETUP
gene expression profiling (Fluidigm Biomark HD)
23
-
-
other
variable
variable
Up to 50x
OTHER
Olink Explore
19.8
-
-
HiFiViral SARS-CoV-2
-
-
-
The liquid handling software that controls the mosquito family of instruments gives users an intuitive experience tailored to their application area.
Developed in collaboration with scientists, the latest mosquito software facilitates miniaturization in novel application areas. Packed with powerful features, the software includes an experiment designer and helpful wizards to create and manage your protocols.