Resources

Explore insightful resources that include videos, whitepapers, eBooks, application notes and more

Precision designed for cell-based workflows - Ebook

View our full ebook on precision designed for cell-based workflows using dragonfly discovery.

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Guide
Precision designed for cell-based workflows - Poster

View our one page on precision designed for cell-based workflows using dragonfly discovery.

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Poster
dragonfly® discovery Precision Dispensing - Ebook

View our full ebook on precision dispensing for every stage of discovery using one platform with multiple workflows and endless possibilities, dragonfly discovery.

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Guide
firefly® technical note SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian

This technical note describes how to run the Takara Bio SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian workflow (Cat. Nos. 634485, 634486, 634487 & 634488) on SPT Labtech’s firefly.

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Technical note
firefly ® technical note Nextera XT DNA Library Preparation (Volume-reduced)

This technical note describes the 5× volume-reduced Illumina Nextera XT DNA Library Preparation workflow on SPT Labtech's firefly. The workflow reduces Nextera XT reagent consumption by five-fold while automating DNA dilution, DNA normalization, tagmentation and indexing, post-tagmentation bead cleanup, library quantification and final library normalization.

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Technical note
firefly ®+ technical note QIAseq® Targeted DNA Panel

Based on the QIAseq Targeted DNA Panel Handbook (06/2025), the workflow is split into two fully automated parts requiring no hands-on intervention.

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Technical note
dragonfly® discovery: Assay development meets uHTS

Ultra-high-throughput screening (uHTS) demands homogeneous assay formats to leverage the density of 1536/3456 well plates to increase the throughput of large compound (> 1 million) library screens against disease targets. Such assay formats require automated reagent dispensing and rigorous assay development. The key liquid handlers in any uHTS system are non-contact reagent dispensers rather than tip-based multichannel pipetting devices that are common in many lower throughput assay systems. Critical requirements for any uHTS reagent dispenser are accuracy, precision, and speed. Additionally, it is important to integrate the uHTS dispenser into assay development as early as possible. For most bulk dispensers, this integration typically occurs late in the process. The dragonfly discovery from SPT Labtech is a non-contact positive displacement dispenser designed to bridge dispensing needs from assay development through high-throughput screening. It provides ready access to 10 different reagents that can be dispensed in variable volumes as well as at high speeds. We evaluate the performance of this dispenser in high-throughput mode and examine its use in assay development for reagent concentration optimization in 1536-well plates and buffer component optimization by DOE.

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Poster
The application of Design of Experiment and the dragonfly discovery to miniaturise, automate and accelerate assay optimization

Automation to carry out complex DoE experiments has always been a limiting factor, both in terms of speed and cost. The dragonfly discovery from SPT Labtech, is a rapid and reliable low volume liquid handler that employs true positive displacement technology to enable the dispensing of all aqueous solutions and solvents, from 200nL through to 4mL. The dragonfly discovery's plate-map file format made it simple to convert and import JMP DoE files and subsequently execute these experimental designs in all microplate types, including 1,536 well plates.

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Poster
dragonfly discovery to enable and accelerate early stage drug discovery processes

The complexity of experimental design in early stage drug discovery is rapidly increasing with reagent requirements for a growing number of assays becoming progressively more complex and difficult to dispense. In order to address these challenges, we have utilized the dragonfly discovery to enable a broad range of applications within functional genomics, phenotypic and biochemical assay workflows.

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Poster
firefly®+ technical note - Illumina DNA PCR free

This technical note provides supporting information for the firefly+ protocols listed below. These protocols are the Illumina DNA PCR free protocols and are available to download from the firefly community. Here, we outline protocol run times, parts required and provide details on the steps performed in each of the firefly+ protocols.

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Technical note
firefly®+ technical note - 10 Genomics' GEM-X 5’ Gene Expression Library Construction

This technical note provides supporting information for automating 10X Genomics' GEM-X 5’ Gene Expression Library Construction (CG000770/CG000733 | Rev A) on SPT Labtech firefly+ liquid handler. These protocols are available to download from the firefly community. Here, we outline protocol run times, parts required and provide details on the steps performed in each protocol.

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Technical note
firefly® technical note - 10x Genomics' GEM-X 5’ Gene Expression Library Construction

This technical note provides supporting information for automating 10X Genomics' GEM-X 5’ Gene Expression Library Construction (CG000770/CG000733 | Rev A) on SPT Labtech firefly liquid handler. These protocols are available to download from the firefly community. Here, we outline protocol run times, parts required and provide details on the steps performed in each protocol.

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Technical note
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