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Sample Prep Series Part 4: Towards Routine Sample Specific Optimization for All
As cryo-EM adoption has grown so has the demand to prepare specimen using conventional commercial instruments and DIY set ups. However, most samples exhibit non-ideal behavior and the resulting specimen rarely achieve the reproducible and...
Sample Prep Series Part 1: Preparing your sample for vitrification
Sample preservation in vitrified ice (vitrification) is widely acknowledged as the first step in the cryogenic electron microscopy (Cryo-EM) workflow. However, prior to sample preparation researchers must consider the sample quality and determine...
Sample Prep Series Part 3: Next Generation Sample Preparation
The period responsible for the current generation of cryogenic electron microscopy (cryo-EM) instrumentation and image processing software is often referred to as the “resolution revolution.” The results of these technological advancements have been...
Sample Prep Series Part 2: Conventional Plunge Vitrification Optimization
The preparation of acceptable frozen-hydrated samples on transmission electron microscopy (TEM) grids is well known to be a challenging bottleneck in the cryogenic electron microscopy (cryo-EM) workflow. During sample vitrification it is assumed...