Liquid handling for hanging drop creation
Fast and repeatable hanging drop protein crystallization, combining experimental flexibility with perfect drop placement.
Standard vapor diffusion
mosquito® can automatically ‘mirror’ drops of crystal screen on a plate
mosquito enables easy addition of extremely low volumes of seed stock and screen solution to the protein drop to enable rapid elucidation of ideal scale-up conditions. mosquito’s ability to multi-aspirate not only provides “in tip” mixing but also gives additional benefits by reducing the minimum dispense volume from 25 nL to 10 nL. Its use of disposable positive displacement tips means that they will never clog when handling the seed stock ‘paste’.
mosquito's unique true positive displacement pipettes combined with its multi-aspirate function allows users to access volumes down to 10 nL. Not only does this save on precious ligands/compounds,
By maintaining a cold environment and pre-chilling the sample holder block it is possible to use mosquito crystal® to accurately and easily pipette the bicelle solution, thereby facilitating the automation of screening for this lipidic based technique of membrane protein crystallization.
The mosquito LCP (SPT Labtech) is very easy to use and user friendly. It is faster and reliable, less maintenance is required. It works like a charm. I am very glad that I bought it for our crystallization lab, especially working with membrane proteins one cannot miss it for LCP setups. It is also very useful for seeding techniques as well
Dr. Chitra Rajendran, Group Leader
Excellent liquid handling for setting up sitting drop crystallography experiments. It was fast! We set up over 4000 experiments with 8 proteins in only 6 hours. I had 27 ‘hits’ for crystallography conditions out of 480 mother liquor conditions.
Dr. James Caldwell, Postdoctoral Research Fellow
SPT Labtech’s mosquito is working well, we have set up a bit over 500 plates since it was installed, and we have had a lot of success with seeding.
Dr. Alastair McEwen, Research Engineer
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