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mosquito

Towards high-throughput low-cost sustainable ecotoxicity testing

Traditional whole animal-based toxicity testing is expensive, time-consuming, and unethical. Furthermore, these tests do not capture the full range of biological impacts. There is growing interest in developing alternative methods (New Approach Methods, NAMs), that are less resource intensive (lower costs, quicker assays, fewer animals), while also providing more data on the mechanism of toxicity. The objective of this work was to optimize and establish a high throughput in vitro transcriptomic-based toxicity testing platform for chemical and environmental risk assessment. The platform couples human Caco-2 and Hep G2 cell lines with QIAGEN’s UPXome ultraplex technology, so that the combined test system can also derive transcriptomic points of departure (tPODs).

Poster

Miniaturization of DNA library preparation for Illumina sequencing using automated positive displacement liquid handling

Advances in next generation sequencing have resulted in huge increases in throughput with associated decreases in costs. As a result, the process of library preparation has become even more of a financial and time constraint to high throughput sequencing core facilities. Automation, in the form of liquid handling robots, has been able to alleviate some of these bottlenecks. This work describes the application of SPT Labtech’s mosquito® HV positive displacement nanoliter liquid handler to successfully implement the preparation of DNA libraries for Illumina sequencing at one tenth the volume of the original manual protocol.

Poster

How to accelerate small molecule lead discovery with automated multichannel pipetting and DEL technology

Establishing an efficient and accurate compound testing workflow is essential to any small molecule screening platform. When utilizing screening methods such as DNA-encoded library (DEL) technology, follow-up hit validation must be performed using traditional biochemical assays. In this webinar, we will review the use of mosquito LV to accelerate early-stage small molecule lead discovery through the lens of Hydroxyacid oxidase 1 (HAO1). In this case study, we will describe our efforts to develop and deploy a robust enzymatic assay towards the development of potent inhibitors identified by DEL technology for disease treatment.

Webinar

mosquito® LV: An Assay’s Best Friend

After a successful DNA-encoded library (DEL) screen, it is essential to have access to a high-throughput method of assaying compounds to validate hits. The ability to create up to sixteen serial dilutions in parallel and subsequently plate those dilutions on a nanoscale can vastly speed up the hit finding process, while improving the accuracy and efficiency of the screen. mosquito® LV by SPT Labtech has greatly improved the way we work with our off-DNA compounds synthesized from DELcore and DELflex collaborations.

Application note

Miniaturisation of DNA library preparation for Illumina sequencing using mosquito® liquid handler

Advances in next generation sequencing have resulted in huge increases in throughput with associated decreases in costs. As a result of these improvements the process of library preparation has become even more of a financial and time constraint to high throughput sequencing core facilities. Automation, in the form of liquid handling robots, has been able to alleviate some of these bottlenecks. This study describes the application of one of these systems, the mosquito HV system, to successfully implement the preparation of DNA libraries for Illumina sequencing in one tenth the volume of the original manual protocol.

Application note

Expanding High Throughput Nanolitre Solution-Phase Crystallisation for Polymorph Screening

Polymorphism is a solid-state phenomenon where a chemical species may adopt different conformational or packing arrangements, therefore allowing for the formation of more than one distinct crystal structure. Crystal polymorphs can exhibit varying physical and chemical properties. These properties such as solubility, hardness, colour, and melting point can all be important in the design of a compound in the crystal engineering and pharmaceutical industries.1, 2 Typically, conducting polymorph screens requires a combination of classical crystallisation techniques which demand both large quantities of material and time to complete. Herein, we describe how encapsulated nanodroplet crystallisation (ENaCt) can be applied for low-quantity rapid polymorph screening using highly polymorphic 5-methyl-2-[(2-nitrophenyl)amino]-3thiophenecarbonitrile (ROY) and nicotinamide as test compounds. ENaCt polymorph screening resulted in the growth, and analysis, of single crystals of a new polymorph of ROY ‘O22’. Crystal forms of both ROY and nicotinamide previously thought to be inaccessible to solution-phase crystallisation were also successfully grown, highlighting the capabilities of ENaCt for screening a diverse polymorphic landscape.

Poster

SLAS 2023 Interview - Chris Waddling - dynamic duo

Discover how genomics researchers can miniaturize NGS methods to increase throughput and lower costs using the combination of the mosquito® and dragonfly® automated liquid handlers from SPT Labtech in this interview with Field Application Scientist, Chris Waddling.

Video

encapsulated nanodroplet crystallisation (ENaCt) for small organic molecules: polymorph discovery

Crystallisation is of vital importance to the drug development pipeline of the pharmaceutical industry due to the existence of multiple crystal forms (e.g. cocrystals, solvates and polymorphs) of an active pharmaceutical ingredient (API). The physical properties of the crystal form of an API are important in manufacturing, API stability and can have significant impact on the rate of API uptake from the solid form (e.g. from a tablet), ultimately influencing both its formulation chemistry and the pharmacokinetics of the API.

Application note

Maximize ROI through miniaturization: How to get more for your money in NGS library preparation

To meet the data-demand of modern genomics research, many new methods and technologies have centered around driving throughput in the laboratory. The advent of miniaturized automated liquid handling in particular has played a significant role, enabling scientists to process samples at a much higher rate, up to hundreds at a time.

Webinar

Expanding High Throughput Nanolitre Solution-Phase Crystallisation for Polymorph Screening

Polymorphism is a solid-state phenomenon where a chemical species may adopt different conformational or packing arrangements, therefore allowing for the formation of more than one distinct crystal structure. Crystal polymorphs can exhibit varying physical and chemicalproperties. These properties such as solubility, hardness, colour, and melting point can all be important in the design of a compound in the crystal engineering and pharmaceutical industries. Typically, conducting polymorph screens requires a combination of classical crystallisation techniques which demand both large quantities of material and time to complete. Herein, we describe how encapsulated nanodroplet crystallisation (ENaCt) can be applied for low-quantity rapid polymorph screening using highly polymorphic 5-methyl-2-[(2-nitrophenyl)amino]-3-thiophenecarbonitrile (ROY) and nicotinamide as test compounds. ENaCt polymorph screening resulted in the growth, and analysis, of single crystals of a new polymorph of ROY ‘O22’. Crystal forms of both ROY and nicotinamide previously thought to be inaccessible to solution-phase crystallisation were also successfully grown, highlighting the capabilities of ENaCt for screening a diverse polymorphic landscape.

Poster

Accurate, miniaturized DNA quantification assays with positive displacement liquid handlers

Quantification and subsequent normalization of nucleic acids are critical steps in many genomics applications. Conventional quantification workflows use high volumes of costly reagents and waste valuable DNA. Furthermore, highly viscous DNA samples pose a challenge for automated liquid handling. Significant increases in throughput together with reduced reagent costs and sample saving can be achieved using the combination of a mosquito® pipetting robot and a dragonfly® discovery dispenser.

Application note

Miniaturised magnetic bead clean‑ups on mosquito® HV genomics

An essential accessory for every NGS lab, the magnetic bead separation block has been optimized for miniaturized SPRI bead clean-ups with mosquito HV genomics. The block and the corresponding mosquito protocol provide a semi-automated process that combines significant cost savings with high recovery rates.

Application note

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