Resources | SPT Labtech

Automating Scale Biosciences’ Single Cell RNA Kit on SPT Labtech’s firefly®

The ability to obtain and map transcripts from single cells has provided significant insight into the fundamentals of how life persists and how disease evolves. ScaleBio enables single cell analysis with an instrument-free method leveraging highly parallelized barcoding, making single cell RNA sequencing more approachable for researchers across both academic and industry spectrums.

Application note

Auto-tRNAseq: Driving throughput in tRNA library generation with firefly®

Transfer RNAs (tRNAs) are the critical adaptor molecules that translate genetic information into functional protein sequences. While the genome defines a static set of tRNA gene sequences, the functional tRNA pool depends on the expression and aminoacylation levels of different tRNA species, as well as many modifications that fine-tune tRNA activity.1,2 Dynamically regulated tRNA pools dictate the fidelity of the translation process and proteome integrity of a cell. Considering the central role of tRNA in translation regulation, investigating the cellular composition of tRNA pools provides a deeper understanding of the translational state of the cell. High-resolution sequencing-based methods for tRNA sequencing have provided transformative insights into the physiological impact of tRNA expression, modification, and aminoacylation levels on cellular stress and/or disease conditions.3–6 More recently, large-scale genome engineering efforts have also employed tRNA sequencing to recapitulate the translational state of engineered cells.

Poster

Elucidating cost-effective miniaturized mRNA-seq library construction and implications for the study of circadian rhythms

Dr. Esser’s group at the University of Florida previously showed that cellular circadian clocks direct a daily transcriptional program that supports homeostasis and resilience. To further learn the age-associated changes in circadian functions, they profiled the circadian transcriptome in different tissues including skeletal muscle, the heart, and others.

Webinar

Combination of scRNA-seq strategies to untangle complex cell populations

In this workshop Dr. Emilio Yángüez López-Cano from the Functional Genomics Center Zurich explains how they select and combine different single cell RNA-seq approaches, adapting them to the requirements of the research project and the complexity of the samples.

Webinar

Taking single-cell RNA-seq by STORM

In this webinar, hear from Van Andel Institute scientists about how the mosquito HV liquid handler was used to miniaturize and increase throughput of the Takara Bio SMART-Seq® Stranded Kit, to yield robust single-cell total RNA libraries from flow-sorted cells, as well as the troubleshooting/optimization challenges and how these were overcome

Webinar

A set of miniaturized high-throughput library protocolsfor RNA sequencing

Here we describe a set of protocols for Illumina library preparation from bulk RNA at miniaturized reaction volumes.

Poster

Automated, low-cost, miniaturized RNA-Seq and DNAseq library prep

Learn more about the needs for next-generation sequencing (NGS), the demand for high-throughput, low-cost, miniaturized sample preparation has increased both in academic and clinical settings.

Poster

Low-volume, automated single cell RNA-seq library preparation with mosquito liquid handlers

Low volume, automated single cell RNA-seq preparation with mosquito liquid handlers are covered in this poster utilizing mosquito HV genomics and mosquito LV genomics instruments.

Poster

Low-volume, single-cell RNA-seq library prep

Low-volume, single-cell RNA-seq library prep: use of mosquito liquid handlers in Nextera XT library preparation and magnetic bead clean up

Application note