firefly displays error messages in red in the execution log, if there is a problem with your protocol or the instrument.
| Error Message | Response |
| Validation errors | |
| This would display the first validation Error as the reason for not executing the protocol | Make sure the Protocol has no errors before trying to execute it |
| Tip change errors | |
| Pipette Head failed to load Tips. | This is usually because tips are already loaded |
| Pipette Head failed to unload Tips. | This is usually because no tips are currently loaded |
| Pipette head errors | |
| Failed to set Pipette Head to volume mode. | Pipette Head error. It may need purging and resetting. You will need access to the manual controls to do this. |
| Failed to set Pipette Head aspirate speed. | Pipette Head error. If this persists, try power cycling your instrument. |
| Failed to aspirate Blowout Volume. | Pipette head error. It may need purging and resetting. You will need access to the manual controls to do this. |
| Failed to aspirate Volume. | Pipette head error. It may need purging and resetting. You will need access to the manual controls to do this. |
| Failed to set Pipette Head dispense speed. | Pipette Head error. If this persists, try power cycling your instrument. |
| Failed to dispense Air Transport Volume. | Pipette head error. It may need purging and resetting. You will need access to the manual controls to do this |
| Failed to dispense Volume. | Pipette head error. It may need purging and resetting. You will need access to the manual controls to do this |
| Failed to dispense Blowout Volume. | Pipette head error. It may need purging and resetting. You will need access to the manual controls to do this |
|
Failed to aspirate Mix Volume.
|
Pipette head error. It may need purging and resetting. You will need access to the manual controls to do this |
| Failed to set Pipette Head dispense speed. | Pipette head error. If this persists, try power cycling your instrument. |
| Failed to dispense Mix Volume. | Pipette head error. It may need purging and resetting. You will need access to the manual controls to do this. |
| Failed to set Pipette Head Post Mix aspirate speed. | Pipette Head error. If this persists, try power cycling your instrument. |
| Failed to aspirate Post Mix Aspirate Volume. | Pipette head error. It may need purging and resetting. You will need access to the manual controls to do this. |
| Failed to dispense Post Mix Aspirate Overshoot Volume. | Pipette head error. It may need purging and resetting. You will need access to the manual controls to do this. |
| Failed to aspirate Post Mix Aspirate Air Transport Volume. | Pipette head error. It may need purging and resetting. You will need access to the manual controls to do this. |
| Failed to set Pipette Head Post Mix dispense speed. | Pipette Head error. If this persists, try power cycling your instrument. |
| Failed to dispense Post Mix Air Transport Volume speed. | Pipette head error. It may need purging and resetting. You will need access to the manual controls to do this. |
| Failed to dispense Post Mix Dispense Volume. | Pipette head error. It may need purging and resetting. You will need access to the manual controls to do this. |
| Failed to dispense Post Mix Blowout Volume. | Pipette head error. It may need purging and resetting. You will need access to the manual controls to do this. |
| Firefly HvHead Error[Aspirate] | Pipette head error during aspirate. May need purging and resetting. You will need access to the manual controls to do this. |
| Firefly HvHead Error[Dispense] | Pipette head error during dispense. May need purging and resetting. You will need access to the manual controls to do this. |
| Dispense head errors | |
| Dispense run cancelled | Dispense head error. Purge syringes of any liquids before continuing. You will need access to the manual controls to do this. |
| Stopping dispense run, due to problem | Dispense head error. Purge syringes of any liquids before continuing. You will need access to the manual controls to do this. |
| Take off | |
| Can't move item: {item name} off stack. No empty locations found | All deck positions are occupied. Was a user interaction to remove a plate missed? |
Contact Reliance (see section 13) if you have a problem with your firefly instrument, as it has no user-repairable parts.
13.1.1 Restarting a protocol from a specific step
If you have had to stop a protocol, you may want to restart from a specific step partway through.
Select the step from the dropdown list. firefly will ask you to confirm your choice.
Before you select Start, check that you are re-starting at a sensible point e.g. you are not about to dispense when there is no liquid aspirated into the syringes.
firefly will ask you to confirm the deck layout before allowing you to restart the protocol
Check that the illustration is correct before restarting, or your protocol will fail again.