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Automated Assay Development

dragonfly® discovery: Assay development meets uHTS

Ultra-high-throughput screening (uHTS) demands homogeneous assay formats to leverage the density of 1536/3456 well plates to increase the throughput of large compound (> 1 million) library screens against disease targets. Such assay formats require automated reagent dispensing and rigorous assay development. The key liquid handlers in any uHTS system are non-contact reagent dispensers rather than tip-based multichannel pipetting devices that are common in many lower throughput assay systems. Critical requirements for any uHTS reagent dispenser are accuracy, precision, and speed. Additionally, it is important to integrate the uHTS dispenser into assay development as early as possible. For most bulk dispensers, this integration typically occurs late in the process. The dragonfly discovery from SPT Labtech is a non-contact positive displacement dispenser designed to bridge dispensing needs from assay development through high-throughput screening. It provides ready access to 10 different reagents that can be dispensed in variable volumes as well as at high speeds. We evaluate the performance of this dispenser in high-throughput mode and examine its use in assay development for reagent concentration optimization in 1536-well plates and buffer component optimization by DOE.

Poster

The application of Design of Experiment and the dragonfly discovery to miniaturise, automate and accelerate assay optimization

Automation to carry out complex DoE experiments has always been a limiting factor, both in terms of speed and cost. The dragonfly discovery from SPT Labtech, is a rapid and reliable low volume liquid handler that employs true positive displacement technology to enable the dispensing of all aqueous solutions and solvents, from 200nL through to 4mL. The dragonfly discovery's plate-map file format made it simple to convert and import JMP DoE files and subsequently execute these experimental designs in all microplate types, including 1,536 well plates.

Poster

dragonfly discovery to enable and accelerate early stage drug discovery processes

The complexity of experimental design in early stage drug discovery is rapidly increasing with reagent requirements for a growing number of assays becoming progressively more complex and difficult to dispense. In order to address these challenges, we have utilized the dragonfly discovery to enable a broad range of applications within functional genomics, phenotypic and biochemical assay workflows.

Poster

Development of a 3D high-content imaging based explorative toxicology platform for tumor and healthy colorectal patient-derived organoids.

This study describes the development of an explorative toxicity screening platform using colorectal PDOs obtained from both tumor and matched healthy tissues. The latter is organized into distinct stem cell and differentiated cell compartments with differing proliferative and functional roles, where stem cells maintain homeostasis and self-renewal, and differentiated cells perform specialized functions including drug uptake and barrier maintenance.

Poster

A design of Experiments Toolbox for Assay Development and Optimization

In this poster we demonstrate that the dragonfly® discovery's accurate, repeatable and rapid low volume transfers, paired with synthace, enabled it to develop a robust RecBCD assay, which offers a versatile toolbox for novel assay optimization

Poster

firefly Demo Overview

Watch as firefly effortlessly streamlines workflows, reducing human error and increasing throughput. With its advanced technology and user-friendly interface, you can focus on your research while firefly takes care of the details. Discover the future of laboratory automation.

Video

Optimizing multi-cellular models for high-throughput screening - Webinar

Join us as we dive into recent research which has combined Design of Experiments methodology with automated liquid handling to develop a cardiotoxicity assay platform. We discuss how this approach could become routine in the future, not only offering more accurate predictability of new drug candidates, but also enabling researchers to leverage resources more efficiently through streamlined workflows and data-driven decision-making.

Webinar

Supercharge your Olink workflow with the right liquid handling tools

In this webinar, discover how to maximize volumetric precision in Olink proteomic technology workflows for improved accuracy and reliability with multi-omic pipelines for full suite service providers. We will discuss how miniaturized liquid handling, enabled by dragonfly® discovery and mosquito® instruments, facilitates high-plex, precise, and repeatable proteomic profiling, supporting exploratory and pre-clinical experiments at Azenta Life Sciences. Insightful process design, in tandem with truly differentiating positive displacement volumetric technologies, enables leading labs to retain flexibility and seamlessly incorporate complex workflows demanding high precision and accuracy. Key learning objectives Understand the value of positive displacement technologies for effective multi-omics assay miniaturization Discover the benefits of incorporating Olink protein profiling technology as an expansion from genomic services into multi-omics Learn more about the practical considerations and applications of performing the Olink assay

Webinar

Automating an Advanced Genomic Sequencing Workflow

Learn about the operation of a sample preparation workflow for advanced genomic sequencing to support the development of personalized cancer vaccines and other next-generation cancer immunotherapies. As an integral part of the sample preparation process, this workflow relies on SPT Labtech’s apricot PP5 multi-channel pipettor and dragonfly® discovery dispensing platform for low-volume reagent dispensing.

Webinar

The Future of Automated Pipetting

In this live event, our panel of experts walked through the challenges that labs face with their liquid handling processes.

Video

Intuitive By Design: SPT Labtech's mosquito® Software

The liquid handling software that controls SPT Labtech's mosquito family of positive displacement pipetting instruments gives users an intuitive experience tailored to their application area. Developed in collaboration with scientists, the latest mosquito software facilitates miniaturization in novel application areas. Packed with powerful features, the software includes an experiment designer and helpful wizards to create and manage your protocols.

Video

CherryPicker II system: A powerful tool for HTS-ADME automated assay

In this poster, we introduce a custom solution for efficiency of HTS-ADME using combined standalone instruments included the newly developed mosquito® X1 Cherry Picker II (CP2)

Poster

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