Traditional approaches to assay development often involve compromise due to limitations of automated liquid handling capability. Frequently resulting in many different and complex experiments, since it is not practical to investigate all assay variables in one plate. Consequently, the scope of assay development/optimization may be significantly limited, for example, with respect to the number of different buffers and component concentrations tested. Liquid handling and experimental design tools often limit the plate density used in assay development to 96 well formats, which are not compatible for HTS.
Here we present the dragonfly discovery liquid handling system for assay development and HTS. This flexible instrument, has been designed specifically for seamless transition between assay development and HTS. In assay development mode, it enables the creation of complex matrix layouts directly in 384 /1,536 well plates.