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A set of miniaturized high-throughput library protocols for RNA sequencing

mosquito HV genomics


Here we describe a set of protocols for Illumina library preparation from bulk RNA at miniaturized reaction volumes. Methods for preparation of long RNA-seq libraries (using either polyadenylated RNA enrichment or rRNA depletion) and small RNA-seq libraries were established on a mosquito pipetting robot that enables accurate liquid transfers at nanoliter to microliter volumes.

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