The automation of protein crystallography screening has contributed significantly to the rapid progress of crystallography-based structural biology offering increased throughput and accuracy, with the ability to use smaller volumes of both protein and screen solutions, thereby saving valuable protein and reducing reagent costs.
Automation of protein crystallization trials set-up requires accurate placement of nanolitre volumes of protein and screen drops, in addition to the reproducible and accurate dispensing of solutions of varying viscosities. This is particularly important for the set-up of the highly viscous lipid mesophases in the LCP crystallization technique for membrane protein crystallization trials.
mosquito® LCP by TTP Labtech offers a fully automated solution to LCP screen set-up. With an optional LCP mixer and a microsyringe dispenser that can accurately dispense nanolitre volumes of the highly viscous cubic phase, mosquito LCP offers fast throughput, high precision and unrivaled reproducibility. mosquito’s positive displacement technology and disposable tips ensure unrivaled accuracy and
zero-contamination between samples.
This poster describes the features of mosquito® crystal and mosquito LCP and their ability to address the issues of the automated set-up of protein crystallization screen trials. The ability to automate both microbatch and vapor diffusion methods of protein crystallography (sitting drop, hanging drop) without instrument configuration change offers significant flexibility for the crystallography laboratory. mosquito LCP offers all the advantages of mosquito crystal but with the addition of a dedicated microsyringe dispenser for accurate dispensing of nanolitre volumes of the highly viscous cubic phase.