If your protocol failed to complete for any reason, before you try and run it again, you should:
- Check the Execution Log for the cause of the problem. If necessary, modify your protocol design or deck layout before you run again.
- Use the Manual Controls to clear firefly's decks and purge and remove tips and syringes.
- Use the Manual Controls to 'Home' firefly, and firefly+. This ensures that the instrument is in the correct state to restart.
- Follow the instructions for restarting from a specific step.
IMPORTANT: if you had run an ODTC pre-method before the protocol stopped, you will need to re-run it before you can run the main method. Restart your firefly protocol so that running the ODTC pre-method is the first step.
- If your protocol utilized the ODTC, you will see the following prompts for restarting a
protocol.- If execution restarts with a Pre method running, you will see guidance for ending it and
-
- If it starts after the Pre method, but before the Main method, you will see:
-
- Once the ODTC is idle and ready to run, the start requirements, i.e. block temperature,
are compared. The state of the hardware is compared to the subsequent main method
incubation starting requirements. If they do not match the protocol will not start e.g.
because the Pre method has not been run as instructed.
- Once the ODTC is idle and ready to run, the start requirements, i.e. block temperature,
-
- The state of the ODTC is also checked, in case it has been switched off i.e. when clearing
up the failed protocol, and is not ready and initialized.
- The state of the ODTC is also checked, in case it has been switched off i.e. when clearing
-
- You will then be ready to rerun the ODTC protocol.
