firefly requires instrument-specific tips, syringes and reservoirs for correct operation. You must always use the correct consumables, which are available from SPT Labtech.
EZL firefly is supplied with a starter kit containing:
- 1 x Standard Reservoirs (pack of 50)
- 1 x LDV Reservoirs (pack of 25)
- 1 x Standard Syringes (pack of 100)
- 1 x ULR Syringes (pack of 100)
- 2 x Racks of 125µL 384 Filter Tips
- 2 x Racks of 125µL 96 Filter Tips
- 2 x Racks of 50µL 384 Filter Tips
- 2 x Racks of 50µL 96 Filter Tips
ATL firefly is supplied with a starter kit containing:
- 1 x Standard Reservoirs (pack of 50)
- 1 x LDV Reservoirs (pack of 25)
- 1 x Standard Syringes (tray of 10)
- 1 x ULR Syringes (tray of 10)
- 2 x Racks of 50µL 96 Filter Tips
- 2 x Racks of 125µL 96 Filter Tips
- 2 x Racks of 50µL 384 Filter Tips
- 2 x Racks of 125µL 384 Filter Tips
3.1.1 EZL tips
Disposable pipetting tips are available in a variety of sizes for working with different plate types.
96 array | 384 array | 8 strips (96 format) | 16 strips (384 format) | |
125µL filtered | sterile | sterile | sterile | sterile |
125µL nonfiltered | sterile | sterile | sterile | sterile |
50µL filtered | sterile | sterile | sterile | sterile |
50µL non-filtered | sterile | sterile | sterile | sterile |
Note that a filtered version has a lower capacity because the filter takes up space (i.e., 125µl filtered is actually 100µl. 50µl filtered is actually 35µl).
Non sterile options of some tip types are available – please enquire.
3.1.2 ATL tips
Disposable ATL pipetting tips are available in the following sizes. The plate on which the firefly+ tips sit in their tip box will be coloured to reflect the tip volume.
- Yellow = 125µL
- Orange = 50µL
96 array | 384 array | |
125µL filtered | sterile | sterile |
125µL non-filtered | sterile | sterile |
50µL filtered | sterile | sterile |
50µL non-filtered | sterile | sterile |
Note that a filtered version has lower capacity because the filter takes up space (i.e.,125µl filtered is actually 100µl, 50µl filtered is actually 35µl).
Non-sterile options of some tip types are available – please enquire.
3.1.3 Tip box adapters
Tip box adapters are required to keep ATL tip boxes rigid while tips are loaded onto the pipetting head. You can load 1-3 columns of tips without an adapter; more than that will require one. firefly software will remind you to use the adapter when required, when designing protocols.
ATL instruments are supplied with tip box adapters; you can purchase more if you find you need
them.